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Adding 2 mL to 50 mg gives a concentration of 25 mg/mL

(2009) Cognitive and psychiatric effects of vitamin B12 replacement in dementia with low serum B12 levels: a nursing home study

ADVANCED NERVOUS SYSTEM SUPPORT: High-dose B12 plays a critical role in neurological health

Modified Irvine, Beatties and Bresnahan forelimb scale (IBB scale, scored 02, 03, 01) (63, 64) for assessment of forelimb function impairment in rats that received cuprizone diet, and then cuprizone intragastrical applications, and thereby, cuprizone toxicity manifestations

Splitting the dose or adding a second daily injection is not part of the clinical protocol and may not improve outcomes

Freezing and Thawing Freezing : Freeze the aliquots quickly by placing them in an ethanol/dry ice bath or a specialized freezer rack to avoid degradation